Review



aavrg ef1a mcherry ires cre  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Addgene inc aavrg ef1a mcherry ires cre
    Aavrg Ef1a Mcherry Ires Cre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 311 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/penn+aav+hsyn+cre+wpre+hgh/pENN%2EAAV%2EhSyn%2ECre%2EWPRE%2EhGH+(Plasmid+%23105553)/pmc13621956-927-0-9
    Average 96 stars, based on 311 article reviews
    aavrg ef1a mcherry ires cre - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Labeling:

    Article Title: A High-Resolution Transcriptomic Atlas of Cell Types in the Ventral Visual Thalamus.
    Article Snippet: .. Monosynaptic labeling of retinorecipient neurons in the vLGN was achieved using bilateral intravitreal injections of pENN.AAV.hSyn.Cre.WPRE.hGH (2.5 × 1013 GC/mL; Addgene viral prep #105553- AAV1; RRID:Addgene_105 553; referred to here as AAV1- Cre). ..

    Injection:

    Article Title: Alarm pheromone activates posterior medial amygdala-to-bed nucleus of the stria terminalis projections in male rats.
    Article Snippet: .. Then, the rats assigned to the hSyn group (n = 9) received a unilateral injection of 0.2 μL of a 1:1 mixture of pAAVCBA-FLEX-SYP-mCherry (produced and packaged in one of our authors’ lab; Uematsu et al., 2017) and pENN-AAV-hSyn-Cre-WPRE-hGH (gifted from Dr. James M. Wilson; Addgene viral prep # 105553-AAV1, htt ps://n2t.net/addgene:105553, RRID: Addgene_105553) directed at the left pvMeA (AP, 3.00; ML, − 1.00; DV, 6.50 mm; Paxinos and Watson, 2007). .. Similarly, a unilateral injection of 0.2 μL of a 1:1 mixture of pAAV-CBA-FLEX-SYP-mCherry (gifted from Dr. Akira Uematsu) and pENN-AAV-CaMKII 0.4-Cre-SV40 (gifted from Dr. James M. Wilson; Addgene viral prep # 105558-AAV1; https://n2t.net/addgene:105558, RRID: Addgene_105558) was directed at the left pvMeA in the rats assigned to the CaMKII group (n = 9).

    Article Title: Co-Conservation of synaptic gene expression and circuitry in collicular neurons
    Article Snippet: .. Four weeks after pENN.AAV.hSyn.Cre.WPRE.hGH (Addgene, Catalog # 105553-AAVrg) was injected into the pulvinar of H2B-TRAP mice, their brains were harvested following rapid decapitation to minimize the effects of stress on gene expression. ..

    Article Title: Targeting the noradrenergic-metabolic axis: A new strategy for sugar-induced depression subtypes.
    Article Snippet: .. We injected 500 nl of pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV retrograde; gifted by Bryan Roth, Addgene viral prep # 44361-AAV Retrograde; RRID: Addgene_44361; titre: 0.625 1013 viral genomes ml 1) into both sides of the PFC (AP: +1.7 mm, ML: ±0.3 mm, DV: 2.3 mm) and 300 nl of pENN-AAV-hSyn-Cre-WPRE-hGH (AAV9; gifted by James M. Wilson, Addgene viral prep # 105553-AAV9; RRID: Addgene_ 105553; titre: 1.15 1013 viral genomes ml 1) into both sides of the LC (AP: 5.4 mm, ML: ±0.8 mm, DV: 3.5 mm). ..

    Article Title: Co-Conservation of synaptic gene expression and circuitry in collicular neurons.
    Article Snippet: .. This identified a total of 982 cluster marker genes that were detected in more than 10% of neurons inside the selected cluster and the minimal outside. snRNA-seq of pulvinar-projecting neurons in mouse SC Four weeks after pENN.AAV.hSyn.Cre.WPRE.hGH (Addgene, Catalog # 105553-AAVrg) was injected into the pulvinar of H2B-TRAP mice, their brains were harvested following rapid decapitation to minimize the effects of stress on gene expression. ..

    Produced:

    Article Title: Alarm pheromone activates posterior medial amygdala-to-bed nucleus of the stria terminalis projections in male rats.
    Article Snippet: .. Then, the rats assigned to the hSyn group (n = 9) received a unilateral injection of 0.2 μL of a 1:1 mixture of pAAVCBA-FLEX-SYP-mCherry (produced and packaged in one of our authors’ lab; Uematsu et al., 2017) and pENN-AAV-hSyn-Cre-WPRE-hGH (gifted from Dr. James M. Wilson; Addgene viral prep # 105553-AAV1, htt ps://n2t.net/addgene:105553, RRID: Addgene_105553) directed at the left pvMeA (AP, 3.00; ML, − 1.00; DV, 6.50 mm; Paxinos and Watson, 2007). .. Similarly, a unilateral injection of 0.2 μL of a 1:1 mixture of pAAV-CBA-FLEX-SYP-mCherry (gifted from Dr. Akira Uematsu) and pENN-AAV-CaMKII 0.4-Cre-SV40 (gifted from Dr. James M. Wilson; Addgene viral prep # 105558-AAV1; https://n2t.net/addgene:105558, RRID: Addgene_105558) was directed at the left pvMeA in the rats assigned to the CaMKII group (n = 9).

    Bioprocessing:

    Article Title: Targeting the noradrenergic-metabolic axis: A new strategy for sugar-induced depression subtypes.
    Article Snippet: .. We thank Addgene for providing the viral vectors pAAV-hSyn-DIO-hM3D (Gq)-mCherry (AAV Retrograde; Addgene viral prep #44361-AAV Ret- rograde; RRID: Addgene_44361) originally deposited by Bryan Roth, and pENN-AAV-hSyn-Cre-WPRE-hGH (AAV9; Addgene viral prep #105553-AAV9; RRID: Addgene_105553) originally deposited by James M. Wilson. ..

    Article Title: Targeting the noradrenergic-metabolic axis: A new strategy for sugar-induced depression subtypes.
    Article Snippet: .. We injected 500 nl of pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV retrograde; gifted by Bryan Roth, Addgene viral prep # 44361-AAV Retrograde; RRID: Addgene_44361; titre: 0.625 1013 viral genomes ml 1) into both sides of the PFC (AP: +1.7 mm, ML: ±0.3 mm, DV: 2.3 mm) and 300 nl of pENN-AAV-hSyn-Cre-WPRE-hGH (AAV9; gifted by James M. Wilson, Addgene viral prep # 105553-AAV9; RRID: Addgene_ 105553; titre: 1.15 1013 viral genomes ml 1) into both sides of the LC (AP: 5.4 mm, ML: ±0.8 mm, DV: 3.5 mm). ..

    Gene Expression:

    Article Title: Co-Conservation of synaptic gene expression and circuitry in collicular neurons
    Article Snippet: .. Four weeks after pENN.AAV.hSyn.Cre.WPRE.hGH (Addgene, Catalog # 105553-AAVrg) was injected into the pulvinar of H2B-TRAP mice, their brains were harvested following rapid decapitation to minimize the effects of stress on gene expression. ..

    Article Title: Co-Conservation of synaptic gene expression and circuitry in collicular neurons.
    Article Snippet: .. This identified a total of 982 cluster marker genes that were detected in more than 10% of neurons inside the selected cluster and the minimal outside. snRNA-seq of pulvinar-projecting neurons in mouse SC Four weeks after pENN.AAV.hSyn.Cre.WPRE.hGH (Addgene, Catalog # 105553-AAVrg) was injected into the pulvinar of H2B-TRAP mice, their brains were harvested following rapid decapitation to minimize the effects of stress on gene expression. ..

    Marker:

    Article Title: Co-Conservation of synaptic gene expression and circuitry in collicular neurons.
    Article Snippet: .. This identified a total of 982 cluster marker genes that were detected in more than 10% of neurons inside the selected cluster and the minimal outside. snRNA-seq of pulvinar-projecting neurons in mouse SC Four weeks after pENN.AAV.hSyn.Cre.WPRE.hGH (Addgene, Catalog # 105553-AAVrg) was injected into the pulvinar of H2B-TRAP mice, their brains were harvested following rapid decapitation to minimize the effects of stress on gene expression. ..

    other:

    Article Title: Co-Conservation of synaptic gene expression and circuitry in collicular neurons.
    Article Snippet: cDNA libraries were then sequenced on an Illumina NextSeq 2000 platform to an average depth of 28,135 reads per cell.



    Similar Products

    aavrg ef1a mcherry ires cre  (Addgene inc)
    96
    Addgene inc aavrg ef1a mcherry ires cre
    Aavrg Ef1a Mcherry Ires Cre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/penn+aav+hsyn+cre+wpre+hgh/pENN%2EAAV%2EhSyn%2ECre%2EWPRE%2EhGH+(Plasmid+%23105553)/pmc13621956-927-0-9
    Average 96 stars, based on 1 article reviews
    aavrg ef1a mcherry ires cre - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    aavrg  (Addgene inc)
    96
    Addgene inc aavrg
    Aavrg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/penn+aav+hsyn+cre+wpre+hgh/pENN%2EAAV%2EhSyn%2ECre%2EWPRE%2EhGH+(Plasmid+%23105553)/pm42771526-340-54-52
    Average 96 stars, based on 1 article reviews
    aavrg - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    aav1 hsyn cre wpre hgh  (Addgene inc)
    96
    Addgene inc aav1 hsyn cre wpre hgh
    Aav1 Hsyn Cre Wpre Hgh, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/penn+aav+hsyn+cre+wpre+hgh/pENN%2EAAV%2EhSyn%2ECre%2EWPRE%2EhGH+(Plasmid+%23105553)/bio_rxiv__64898__2026__03__27__714842-215-5-6
    Average 96 stars, based on 1 article reviews
    aav1 hsyn cre wpre hgh - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    addgene 105553 2e11 gc ml  (Addgene inc)
    96
    Addgene inc addgene 105553 2e11 gc ml
    Addgene 105553 2e11 Gc Ml, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/penn+aav+hsyn+cre+wpre+hgh/pENN%2EAAV%2EhSyn%2ECre%2EWPRE%2EhGH+(Plasmid+%23105553)/bio_rxiv__64898__2026__03__14__711800-246-39-39
    Average 96 stars, based on 1 article reviews
    addgene 105553 2e11 gc ml - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    aav rg hsyn1 egfp  (Addgene inc)
    96
    Addgene inc aav rg hsyn1 egfp
    (A) Schematic of retrograde labeling of input projection neurons to the VTA using <t>AAV.rg-hSyn1-EGFP.</t> (B) Micrograph of an exemplary coronal brain section upon machine learning-assisted brain region mapping (Aligning Big Brains & Atlases (ABBA/ImageJ)). Back-labeled neurons are highlighted in the mPFC and LHA. (C) Circos plot showing relative input strength from reward-related regions to the VTA comparing eBED-prone versus-resilient Tet1 +/– mice (upper panel); corresponding binge sizes of individual Tet1 +/- mice across 5 eBED days plotted on a Log 10 scale to visualize varied responses (lower panel; shaded area shows normal binge response). Data are presented as mean minimum to maximum values. ** P < 0.01. n = 4 mice (unpaired Student’s t -test). (D) Coronal brain schematics of mPFC subregions ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). (E) Representative micrographs of mPFC PL from eBED-prone versus-resilient Tet1 +/− mice. Scale bar 100 μm. (F) Bar graphs of raw EGFP density values from mPFC subregions hemisections ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). Data are presented as mean ± SEM. * P < 0.05. n = 4 mice (two-tailed unpaired Student’s t -test). (G) Schematic illustrating chemogenetic approach to selectively inhibit VTA-projecting mPFC PL neurons using a dual AAV approach (AAV.rg-Cre + AAV-hSyn1-FLEX-hM4Di-mCherry). (H) Micrograph of VTA-projecting mPFC PL neurons expressing mCherry (red) intermingled with layer-5 pyramidal neurons (CTIP2: grey). Scale bar: 500 μm and 50 μm (insert). (I) Cumulative binge intake of hM4Di mPFC-VTA mice and controls per individual days (left panel). Data are presented as mean ± SEM. *** P < 0.001, **** P < 0.0001. n = 4–5 mice (multiple unpaired Student’s t -test); mean binge sizes of hM4Di mPFC-VTA mice and controls (right panel). Data are presented as mean and minimum to maximum. * P < 0.05, n = 4–5 mice (one-tailed unpaired Student’s t -test).
    Aav Rg Hsyn1 Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/penn+aav+hsyn+cre+wpre+hgh/pENN%2EAAV%2EhSyn%2ECre%2EWPRE%2EhGH+(Plasmid+%23105553)/bio_rxiv__64898__2026__03__14__711800-250-7-10
    Average 96 stars, based on 1 article reviews
    aav rg hsyn1 egfp - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    penn aav hsyn cre wpre hgh  (Addgene inc)
    96
    Addgene inc penn aav hsyn cre wpre hgh
    (A) Schematic of retrograde labeling of input projection neurons to the VTA using <t>AAV.rg-hSyn1-EGFP.</t> (B) Micrograph of an exemplary coronal brain section upon machine learning-assisted brain region mapping (Aligning Big Brains & Atlases (ABBA/ImageJ)). Back-labeled neurons are highlighted in the mPFC and LHA. (C) Circos plot showing relative input strength from reward-related regions to the VTA comparing eBED-prone versus-resilient Tet1 +/– mice (upper panel); corresponding binge sizes of individual Tet1 +/- mice across 5 eBED days plotted on a Log 10 scale to visualize varied responses (lower panel; shaded area shows normal binge response). Data are presented as mean minimum to maximum values. ** P < 0.01. n = 4 mice (unpaired Student’s t -test). (D) Coronal brain schematics of mPFC subregions ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). (E) Representative micrographs of mPFC PL from eBED-prone versus-resilient Tet1 +/− mice. Scale bar 100 μm. (F) Bar graphs of raw EGFP density values from mPFC subregions hemisections ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). Data are presented as mean ± SEM. * P < 0.05. n = 4 mice (two-tailed unpaired Student’s t -test). (G) Schematic illustrating chemogenetic approach to selectively inhibit VTA-projecting mPFC PL neurons using a dual AAV approach (AAV.rg-Cre + AAV-hSyn1-FLEX-hM4Di-mCherry). (H) Micrograph of VTA-projecting mPFC PL neurons expressing mCherry (red) intermingled with layer-5 pyramidal neurons (CTIP2: grey). Scale bar: 500 μm and 50 μm (insert). (I) Cumulative binge intake of hM4Di mPFC-VTA mice and controls per individual days (left panel). Data are presented as mean ± SEM. *** P < 0.001, **** P < 0.0001. n = 4–5 mice (multiple unpaired Student’s t -test); mean binge sizes of hM4Di mPFC-VTA mice and controls (right panel). Data are presented as mean and minimum to maximum. * P < 0.05, n = 4–5 mice (one-tailed unpaired Student’s t -test).
    Penn Aav Hsyn Cre Wpre Hgh, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/penn+aav+hsyn+cre+wpre+hgh/pENN%2EAAV%2EhSyn%2ECre%2EWPRE%2EhGH+(Plasmid+%23105553)/pm41814933-51-15-20
    Average 96 stars, based on 1 article reviews
    penn aav hsyn cre wpre hgh - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    james m wilson  (Addgene inc)
    96
    Addgene inc james m wilson
    (A) Schematic of retrograde labeling of input projection neurons to the VTA using <t>AAV.rg-hSyn1-EGFP.</t> (B) Micrograph of an exemplary coronal brain section upon machine learning-assisted brain region mapping (Aligning Big Brains & Atlases (ABBA/ImageJ)). Back-labeled neurons are highlighted in the mPFC and LHA. (C) Circos plot showing relative input strength from reward-related regions to the VTA comparing eBED-prone versus-resilient Tet1 +/– mice (upper panel); corresponding binge sizes of individual Tet1 +/- mice across 5 eBED days plotted on a Log 10 scale to visualize varied responses (lower panel; shaded area shows normal binge response). Data are presented as mean minimum to maximum values. ** P < 0.01. n = 4 mice (unpaired Student’s t -test). (D) Coronal brain schematics of mPFC subregions ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). (E) Representative micrographs of mPFC PL from eBED-prone versus-resilient Tet1 +/− mice. Scale bar 100 μm. (F) Bar graphs of raw EGFP density values from mPFC subregions hemisections ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). Data are presented as mean ± SEM. * P < 0.05. n = 4 mice (two-tailed unpaired Student’s t -test). (G) Schematic illustrating chemogenetic approach to selectively inhibit VTA-projecting mPFC PL neurons using a dual AAV approach (AAV.rg-Cre + AAV-hSyn1-FLEX-hM4Di-mCherry). (H) Micrograph of VTA-projecting mPFC PL neurons expressing mCherry (red) intermingled with layer-5 pyramidal neurons (CTIP2: grey). Scale bar: 500 μm and 50 μm (insert). (I) Cumulative binge intake of hM4Di mPFC-VTA mice and controls per individual days (left panel). Data are presented as mean ± SEM. *** P < 0.001, **** P < 0.0001. n = 4–5 mice (multiple unpaired Student’s t -test); mean binge sizes of hM4Di mPFC-VTA mice and controls (right panel). Data are presented as mean and minimum to maximum. * P < 0.05, n = 4–5 mice (one-tailed unpaired Student’s t -test).
    James M Wilson, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/penn+aav+hsyn+cre+wpre+hgh/pENN%2EAAV%2EhSyn%2ECre%2EWPRE%2EhGH+(Plasmid+%23105553)/bio_rxiv__64898__2026__03__08__708251-187-10-13
    Average 96 stars, based on 1 article reviews
    james m wilson - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    aav hsyn cre  (Addgene inc)
    96
    Addgene inc aav hsyn cre
    (A) Schematic of retrograde labeling of input projection neurons to the VTA using <t>AAV.rg-hSyn1-EGFP.</t> (B) Micrograph of an exemplary coronal brain section upon machine learning-assisted brain region mapping (Aligning Big Brains & Atlases (ABBA/ImageJ)). Back-labeled neurons are highlighted in the mPFC and LHA. (C) Circos plot showing relative input strength from reward-related regions to the VTA comparing eBED-prone versus-resilient Tet1 +/– mice (upper panel); corresponding binge sizes of individual Tet1 +/- mice across 5 eBED days plotted on a Log 10 scale to visualize varied responses (lower panel; shaded area shows normal binge response). Data are presented as mean minimum to maximum values. ** P < 0.01. n = 4 mice (unpaired Student’s t -test). (D) Coronal brain schematics of mPFC subregions ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). (E) Representative micrographs of mPFC PL from eBED-prone versus-resilient Tet1 +/− mice. Scale bar 100 μm. (F) Bar graphs of raw EGFP density values from mPFC subregions hemisections ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). Data are presented as mean ± SEM. * P < 0.05. n = 4 mice (two-tailed unpaired Student’s t -test). (G) Schematic illustrating chemogenetic approach to selectively inhibit VTA-projecting mPFC PL neurons using a dual AAV approach (AAV.rg-Cre + AAV-hSyn1-FLEX-hM4Di-mCherry). (H) Micrograph of VTA-projecting mPFC PL neurons expressing mCherry (red) intermingled with layer-5 pyramidal neurons (CTIP2: grey). Scale bar: 500 μm and 50 μm (insert). (I) Cumulative binge intake of hM4Di mPFC-VTA mice and controls per individual days (left panel). Data are presented as mean ± SEM. *** P < 0.001, **** P < 0.0001. n = 4–5 mice (multiple unpaired Student’s t -test); mean binge sizes of hM4Di mPFC-VTA mice and controls (right panel). Data are presented as mean and minimum to maximum. * P < 0.05, n = 4–5 mice (one-tailed unpaired Student’s t -test).
    Aav Hsyn Cre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/penn+aav+hsyn+cre+wpre+hgh/pENN%2EAAV%2EhSyn%2ECre%2EWPRE%2EhGH+(Plasmid+%23105553)/10__1016_slash_j__cub__2026__02__058-213-32-40
    Average 96 stars, based on 1 article reviews
    aav hsyn cre - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    aavrg hsyn cre  (Addgene inc)
    96
    Addgene inc aavrg hsyn cre
    (A) Schematic of retrograde labeling of input projection neurons to the VTA using <t>AAV.rg-hSyn1-EGFP.</t> (B) Micrograph of an exemplary coronal brain section upon machine learning-assisted brain region mapping (Aligning Big Brains & Atlases (ABBA/ImageJ)). Back-labeled neurons are highlighted in the mPFC and LHA. (C) Circos plot showing relative input strength from reward-related regions to the VTA comparing eBED-prone versus-resilient Tet1 +/– mice (upper panel); corresponding binge sizes of individual Tet1 +/- mice across 5 eBED days plotted on a Log 10 scale to visualize varied responses (lower panel; shaded area shows normal binge response). Data are presented as mean minimum to maximum values. ** P < 0.01. n = 4 mice (unpaired Student’s t -test). (D) Coronal brain schematics of mPFC subregions ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). (E) Representative micrographs of mPFC PL from eBED-prone versus-resilient Tet1 +/− mice. Scale bar 100 μm. (F) Bar graphs of raw EGFP density values from mPFC subregions hemisections ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). Data are presented as mean ± SEM. * P < 0.05. n = 4 mice (two-tailed unpaired Student’s t -test). (G) Schematic illustrating chemogenetic approach to selectively inhibit VTA-projecting mPFC PL neurons using a dual AAV approach (AAV.rg-Cre + AAV-hSyn1-FLEX-hM4Di-mCherry). (H) Micrograph of VTA-projecting mPFC PL neurons expressing mCherry (red) intermingled with layer-5 pyramidal neurons (CTIP2: grey). Scale bar: 500 μm and 50 μm (insert). (I) Cumulative binge intake of hM4Di mPFC-VTA mice and controls per individual days (left panel). Data are presented as mean ± SEM. *** P < 0.001, **** P < 0.0001. n = 4–5 mice (multiple unpaired Student’s t -test); mean binge sizes of hM4Di mPFC-VTA mice and controls (right panel). Data are presented as mean and minimum to maximum. * P < 0.05, n = 4–5 mice (one-tailed unpaired Student’s t -test).
    Aavrg Hsyn Cre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/penn+aav+hsyn+cre+wpre+hgh/pENN%2EAAV%2EhSyn%2ECre%2EWPRE%2EhGH+(Plasmid+%23105553)/pmc13007250-378-12-13
    Average 96 stars, based on 1 article reviews
    aavrg hsyn cre - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Schematic of retrograde labeling of input projection neurons to the VTA using AAV.rg-hSyn1-EGFP. (B) Micrograph of an exemplary coronal brain section upon machine learning-assisted brain region mapping (Aligning Big Brains & Atlases (ABBA/ImageJ)). Back-labeled neurons are highlighted in the mPFC and LHA. (C) Circos plot showing relative input strength from reward-related regions to the VTA comparing eBED-prone versus-resilient Tet1 +/– mice (upper panel); corresponding binge sizes of individual Tet1 +/- mice across 5 eBED days plotted on a Log 10 scale to visualize varied responses (lower panel; shaded area shows normal binge response). Data are presented as mean minimum to maximum values. ** P < 0.01. n = 4 mice (unpaired Student’s t -test). (D) Coronal brain schematics of mPFC subregions ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). (E) Representative micrographs of mPFC PL from eBED-prone versus-resilient Tet1 +/− mice. Scale bar 100 μm. (F) Bar graphs of raw EGFP density values from mPFC subregions hemisections ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). Data are presented as mean ± SEM. * P < 0.05. n = 4 mice (two-tailed unpaired Student’s t -test). (G) Schematic illustrating chemogenetic approach to selectively inhibit VTA-projecting mPFC PL neurons using a dual AAV approach (AAV.rg-Cre + AAV-hSyn1-FLEX-hM4Di-mCherry). (H) Micrograph of VTA-projecting mPFC PL neurons expressing mCherry (red) intermingled with layer-5 pyramidal neurons (CTIP2: grey). Scale bar: 500 μm and 50 μm (insert). (I) Cumulative binge intake of hM4Di mPFC-VTA mice and controls per individual days (left panel). Data are presented as mean ± SEM. *** P < 0.001, **** P < 0.0001. n = 4–5 mice (multiple unpaired Student’s t -test); mean binge sizes of hM4Di mPFC-VTA mice and controls (right panel). Data are presented as mean and minimum to maximum. * P < 0.05, n = 4–5 mice (one-tailed unpaired Student’s t -test).

    Journal: bioRxiv

    Article Title: Midbrain Tet1 dosage defines inter-individual binge-eating susceptibility

    doi: 10.64898/2026.03.14.711800

    Figure Lengend Snippet: (A) Schematic of retrograde labeling of input projection neurons to the VTA using AAV.rg-hSyn1-EGFP. (B) Micrograph of an exemplary coronal brain section upon machine learning-assisted brain region mapping (Aligning Big Brains & Atlases (ABBA/ImageJ)). Back-labeled neurons are highlighted in the mPFC and LHA. (C) Circos plot showing relative input strength from reward-related regions to the VTA comparing eBED-prone versus-resilient Tet1 +/– mice (upper panel); corresponding binge sizes of individual Tet1 +/- mice across 5 eBED days plotted on a Log 10 scale to visualize varied responses (lower panel; shaded area shows normal binge response). Data are presented as mean minimum to maximum values. ** P < 0.01. n = 4 mice (unpaired Student’s t -test). (D) Coronal brain schematics of mPFC subregions ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). (E) Representative micrographs of mPFC PL from eBED-prone versus-resilient Tet1 +/− mice. Scale bar 100 μm. (F) Bar graphs of raw EGFP density values from mPFC subregions hemisections ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). Data are presented as mean ± SEM. * P < 0.05. n = 4 mice (two-tailed unpaired Student’s t -test). (G) Schematic illustrating chemogenetic approach to selectively inhibit VTA-projecting mPFC PL neurons using a dual AAV approach (AAV.rg-Cre + AAV-hSyn1-FLEX-hM4Di-mCherry). (H) Micrograph of VTA-projecting mPFC PL neurons expressing mCherry (red) intermingled with layer-5 pyramidal neurons (CTIP2: grey). Scale bar: 500 μm and 50 μm (insert). (I) Cumulative binge intake of hM4Di mPFC-VTA mice and controls per individual days (left panel). Data are presented as mean ± SEM. *** P < 0.001, **** P < 0.0001. n = 4–5 mice (multiple unpaired Student’s t -test); mean binge sizes of hM4Di mPFC-VTA mice and controls (right panel). Data are presented as mean and minimum to maximum. * P < 0.05, n = 4–5 mice (one-tailed unpaired Student’s t -test).

    Article Snippet: To perform brain-wide mapping of VTA inputs, AAV rg -hSyn1-EGFP (Addgene, #105553, 7E12 GC/mL) was bilaterally injected into the VTA of 6–8-week-old Tet1 +/– mice and littermate controls.

    Techniques: Labeling, Two Tailed Test, Expressing, One-tailed Test